Chromatin organization and gene expression are interdependent. Previously, our group had showed that nucleosomes form groups, termed clutches, whose size correlates with cell state. In this paper, Alvaro, together with Jason Otterstrom, carried out 2-color super-resolution microscopy to image core histones and DNA at nanoscale resolution. They identified the “clutch DNA” as the DNA whose compaction is dependent on the clutch. Moreover, they studied how the epigenetic state of the clutch alters the radius of the clutch DNA.These results provide novel insights into chromatin organization at the nanoscale level and show how the clutches affect DNA packaging.
Castells-Garcia et al. 2019 Nucleic Acids Research
Martina, Ruben and Pia discuss in this State-of-the-Art Review of The FEBS Journal the latest advances and applications of in vivo reprogramming strategies for regenerative medicine with a special focus on dedifferentiation, transdifferentiation and cell fusion processes. Cover of The FEBS Journal
Scientific collaboration between GIBH and CRG: 2 Postdoctoral Positions to study stem cell physiology and somatic cell reprogramming at the Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, China
In GenStorm our goal is to combine high resolution microscopy with molecular modeling techniques. With this multidisciplinary approach we aim to understand how genes are organized within the cell nucleus and how this affects their function. The project is coordinated by Victoria Neguembor (Cosma lab at CRG) and Pablo Dans (Orozco lab at IRBB).