Chromatin organization and gene expression are interdependent. Previously, our group had showed that nucleosomes form groups, termed clutches, whose size correlates with cell state.
In this paper, Alvaro, together with Jason Otterstrom, carried out 2-color super-resolution microscopy to image core histones and DNA at nanoscale resolution.
They identified the “clutch DNA” as the DNA whose compaction is dependent on the clutch. Moreover, they studied how the epigenetic state of the clutch alters the radius of the clutch DNA.
These results provide novel insights into chromatin organization at the nanoscale level and show how the clutches affect DNA packaging.