Dissecting out how Wnt signalling controls somatic cell reprogramming.
We have shown that cyclic activation of the Wnt/b-catenin signalling pathway enhances cell-fusion-mediated reprogramming of a variety of somatic cells (Lluis et al., Cell Stem Cell 2008, Marucci et al. Cell Reports 2014). Tcf3 and Tcf1 are two key effectors in the pathway, and are essential in this process (Lluis et al., PNAS 2011; Ombrato et al., Cell Cycle 2012; Aulicino et al., Stem Cell Report 2014).
Currently we are studying how the dynamics of the Wnt pathway maintains cell pluripotency, and we are reconstructing the regulatory network that is controlled by b-catenin/Tcf factors that have pivotal roles in the regulation of pluripotency in embryonic stem cells and somatic cell reprogramming.